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clonepix2 robot  (Molecular Devices LLC)


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    Structured Review

    Molecular Devices LLC clonepix2 robot
    (A) G418 selected colonies visible in a single 35mm well illuminated with white light at 6 days. (B) The same single 35mm well illuminated with 490 nm wavelength light. Colonies actively secreting rgp120 have a green “halo” visible at 525 nm. (C) Relative mean exterior fluorescence of halo for more than 10,000 colonies imaged by the <t>ClonePix2</t> plotted by rank. The top ranking 0.1% of colonies (44) were robotically picked and cultured. The six clones expressing 0.2–0.4 g/L at day 56 are shown in red.
    Clonepix2 Robot, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 93/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/clonepix2 robot/product/Molecular Devices LLC
    Average 93 stars, based on 50 article reviews
    clonepix2 robot - by Bioz Stars, 2026-06
    93/100 stars

    Images

    1) Product Images from "Robotic selection for the rapid development of stable CHO cell lines for HIV vaccine production"

    Article Title: Robotic selection for the rapid development of stable CHO cell lines for HIV vaccine production

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0197656

    (A) G418 selected colonies visible in a single 35mm well illuminated with white light at 6 days. (B) The same single 35mm well illuminated with 490 nm wavelength light. Colonies actively secreting rgp120 have a green “halo” visible at 525 nm. (C) Relative mean exterior fluorescence of halo for more than 10,000 colonies imaged by the ClonePix2 plotted by rank. The top ranking 0.1% of colonies (44) were robotically picked and cultured. The six clones expressing 0.2–0.4 g/L at day 56 are shown in red.
    Figure Legend Snippet: (A) G418 selected colonies visible in a single 35mm well illuminated with white light at 6 days. (B) The same single 35mm well illuminated with 490 nm wavelength light. Colonies actively secreting rgp120 have a green “halo” visible at 525 nm. (C) Relative mean exterior fluorescence of halo for more than 10,000 colonies imaged by the ClonePix2 plotted by rank. The top ranking 0.1% of colonies (44) were robotically picked and cultured. The six clones expressing 0.2–0.4 g/L at day 56 are shown in red.

    Techniques Used: Fluorescence, Cell Culture, Clone Assay, Expressing

    Six stable MGAT1 - CHO cell lines identified with the ClonePix2 were selected as potential substrates for HIV vaccine production. (A) Immunoblot of affinity-purified rgp120 (50 ng per lane) produced by each of six A244_N332-rgp120 cell lines: 3E, 5C, 5D, 3F, 6B, and 5F. Purified A244_N332-rgp120 produced in normal CHO DG44 cells (692) was shown for purpose of comparison. (B) Comparison of A244_N332-rgp120 protein yields as determined by ELISA from the six MGAT1 - CHO cell lines. ( C) SDS PAGE of rgp120 produced by the 5F MGAT1 - CHO cell line. Supernatant samples (10 μ l per lane) collected over the time course of the culture were electrophoresed on a 4–12% NuPage PAGE SDS gel in MOPS buffer (Thermo Scientific, Waltham, MA). The gel was stained with Simply Blue (Thermo Scientific, Waltham, MA) and visualized using an Innotech FluoChem2 system (Genetic Technologies, Grover, MO).
    Figure Legend Snippet: Six stable MGAT1 - CHO cell lines identified with the ClonePix2 were selected as potential substrates for HIV vaccine production. (A) Immunoblot of affinity-purified rgp120 (50 ng per lane) produced by each of six A244_N332-rgp120 cell lines: 3E, 5C, 5D, 3F, 6B, and 5F. Purified A244_N332-rgp120 produced in normal CHO DG44 cells (692) was shown for purpose of comparison. (B) Comparison of A244_N332-rgp120 protein yields as determined by ELISA from the six MGAT1 - CHO cell lines. ( C) SDS PAGE of rgp120 produced by the 5F MGAT1 - CHO cell line. Supernatant samples (10 μ l per lane) collected over the time course of the culture were electrophoresed on a 4–12% NuPage PAGE SDS gel in MOPS buffer (Thermo Scientific, Waltham, MA). The gel was stained with Simply Blue (Thermo Scientific, Waltham, MA) and visualized using an Innotech FluoChem2 system (Genetic Technologies, Grover, MO).

    Techniques Used: Western Blot, Affinity Purification, Produced, Purification, Enzyme-linked Immunosorbent Assay, SDS Page, SDS-Gel, Staining



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    (A) G418 selected colonies visible in a single 35mm well illuminated with white light at 6 days. (B) The same single 35mm well illuminated with 490 nm wavelength light. Colonies actively secreting rgp120 have a green “halo” visible at 525 nm. (C) Relative mean exterior fluorescence of halo for more than 10,000 colonies imaged by the <t>ClonePix2</t> plotted by rank. The top ranking 0.1% of colonies (44) were robotically picked and cultured. The six clones expressing 0.2–0.4 g/L at day 56 are shown in red.
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    Image Search Results


    (A) G418 selected colonies visible in a single 35mm well illuminated with white light at 6 days. (B) The same single 35mm well illuminated with 490 nm wavelength light. Colonies actively secreting rgp120 have a green “halo” visible at 525 nm. (C) Relative mean exterior fluorescence of halo for more than 10,000 colonies imaged by the ClonePix2 plotted by rank. The top ranking 0.1% of colonies (44) were robotically picked and cultured. The six clones expressing 0.2–0.4 g/L at day 56 are shown in red.

    Journal: PLoS ONE

    Article Title: Robotic selection for the rapid development of stable CHO cell lines for HIV vaccine production

    doi: 10.1371/journal.pone.0197656

    Figure Lengend Snippet: (A) G418 selected colonies visible in a single 35mm well illuminated with white light at 6 days. (B) The same single 35mm well illuminated with 490 nm wavelength light. Colonies actively secreting rgp120 have a green “halo” visible at 525 nm. (C) Relative mean exterior fluorescence of halo for more than 10,000 colonies imaged by the ClonePix2 plotted by rank. The top ranking 0.1% of colonies (44) were robotically picked and cultured. The six clones expressing 0.2–0.4 g/L at day 56 are shown in red.

    Article Snippet: Immunoaffinity purified anti-gp120 for use in the ClonePix2 robot (Molecular Devices, Sunnyvale, CA) was isolated from goat sera by immunoaffinity chromatography using a column consisting of gp120 coupled to Sepharose 4B (GE Healthcare, Little Chalfont, United Kingdom).

    Techniques: Fluorescence, Cell Culture, Clone Assay, Expressing

    Six stable MGAT1 - CHO cell lines identified with the ClonePix2 were selected as potential substrates for HIV vaccine production. (A) Immunoblot of affinity-purified rgp120 (50 ng per lane) produced by each of six A244_N332-rgp120 cell lines: 3E, 5C, 5D, 3F, 6B, and 5F. Purified A244_N332-rgp120 produced in normal CHO DG44 cells (692) was shown for purpose of comparison. (B) Comparison of A244_N332-rgp120 protein yields as determined by ELISA from the six MGAT1 - CHO cell lines. ( C) SDS PAGE of rgp120 produced by the 5F MGAT1 - CHO cell line. Supernatant samples (10 μ l per lane) collected over the time course of the culture were electrophoresed on a 4–12% NuPage PAGE SDS gel in MOPS buffer (Thermo Scientific, Waltham, MA). The gel was stained with Simply Blue (Thermo Scientific, Waltham, MA) and visualized using an Innotech FluoChem2 system (Genetic Technologies, Grover, MO).

    Journal: PLoS ONE

    Article Title: Robotic selection for the rapid development of stable CHO cell lines for HIV vaccine production

    doi: 10.1371/journal.pone.0197656

    Figure Lengend Snippet: Six stable MGAT1 - CHO cell lines identified with the ClonePix2 were selected as potential substrates for HIV vaccine production. (A) Immunoblot of affinity-purified rgp120 (50 ng per lane) produced by each of six A244_N332-rgp120 cell lines: 3E, 5C, 5D, 3F, 6B, and 5F. Purified A244_N332-rgp120 produced in normal CHO DG44 cells (692) was shown for purpose of comparison. (B) Comparison of A244_N332-rgp120 protein yields as determined by ELISA from the six MGAT1 - CHO cell lines. ( C) SDS PAGE of rgp120 produced by the 5F MGAT1 - CHO cell line. Supernatant samples (10 μ l per lane) collected over the time course of the culture were electrophoresed on a 4–12% NuPage PAGE SDS gel in MOPS buffer (Thermo Scientific, Waltham, MA). The gel was stained with Simply Blue (Thermo Scientific, Waltham, MA) and visualized using an Innotech FluoChem2 system (Genetic Technologies, Grover, MO).

    Article Snippet: Immunoaffinity purified anti-gp120 for use in the ClonePix2 robot (Molecular Devices, Sunnyvale, CA) was isolated from goat sera by immunoaffinity chromatography using a column consisting of gp120 coupled to Sepharose 4B (GE Healthcare, Little Chalfont, United Kingdom).

    Techniques: Western Blot, Affinity Purification, Produced, Purification, Enzyme-linked Immunosorbent Assay, SDS Page, SDS-Gel, Staining